重囗另类BBWSeⅹHD,av狼论坛,精品一卡2卡三卡4卡乱码理论,体育生gv老师浪小辉3p警察

Specific Expression of Maize SBEIIb

時間:2023-04-28 18:19:39 工業農業論文 我要投稿
  • 相關推薦

Specific Expression of Maize SBEIIb Promoter Mediated by Different Promoter Region in Transgenic Tobacco Plants

Starch branching enzyme (SBE) catalyzes the biosynthesis of amylopectin. We described the isolation and characterization of SBEIIb promoter and their expression patterns in transgenic tobacco. Using the genomic DNA of maize cultivar Lunuo 1 as template, the SBEIIb promoter was isolated by PCR and was cloned into pMD18-T vector. To study SEBIIb gene regulation at the cellular level, SBEIIb promoter was fused to the β-glucuronidase (GUS) report gene. The results of the fluorometric GUS assays indicate that the sbeIIb-GUS fusion directed a seed-specific expression. Four series of constructs were made with the promoter and the GUS reporter gene to investigate the cis-acting analysis, showing that the four different constructs all can drive expression of the GUS gene in seed plumule and cotyledon and the GUS activity was apparently decreased with the progressive loss of promoter 5' end.

作 者: SUN Cui-xia HAN Jing LI Meng WANG Xiao-peng ZHANG Guo-dong TIAN Yan-chen WANG Ze-li   作者單位: SUN Cui-xia,LI Meng,WANG Xiao-peng,ZHANG Guo-dong,TIAN Yan-chen,WANG Ze-li(National Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, P.R.China)

HAN Jing(Maize Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250000, P.R. China) 

刊 名: 中國農業科學(英文版)  英文刊名: AGRICULTURAL SCIENCES IN CHINA  年,卷(期): 2009 8(11)  分類號: S1  關鍵詞: maize starch-branching enzyme   promoter   cloning   specific expression  

【Specific Expression of Maize SBEIIb 】相關文章:

Induction of defence gene expression by oligogalacturonic acid requires increases in both cytosolic calcium and hydrogen04-26

Induction of defence gene expression by oligogalacturonic acid requires increases in both cytosolic calcium and hydrogen04-26

Down-Regulated Expression of RACK1 Gene by RNA Interference Enhances Drought Tolerance in Rice04-26

Detection of CD2 expression in chicken hematogenic embryo yolk sac lymphoid cells prior to thymus genesis04-26

主站蜘蛛池模板: 从化市| 南木林县| 临洮县| 沐川县| 清徐县| 赤壁市| 民乐县| 屏东市| 凉城县| 平陆县| 安溪县| 定安县| 道孚县| 阜康市| 图木舒克市| 昌图县| 如东县| 卢湾区| 临安市| 临江市| 怀仁县| 轮台县| 迭部县| 红河县| 马龙县| 汝城县| 即墨市| 无锡市| 山西省| 拜城县| 马尔康县| 沙洋县| 厦门市| 晋城| 肃宁县| 堆龙德庆县| 应城市| 赞皇县| 青海省| 齐齐哈尔市| 延川县|