Epigenetic reprogramming in mammalian nuclear transfer

Somatic cloning has been succeeded in some species, but the cloning efficiency is very low, which limits the application of the technique in many areas of research and biotechnology. The cloning of mammals by somatic cell nuclear transfer (NT) requires epigenetic reprogramming of the differentiated state of donor cell to a totipotent, embryonic ground state. Accumulating evidence indicates that incomplete or inappropriate epigenetic reprogramming of donor nuclei is likely to be the primary cause of failures in nuclear transfer. This review summarizes the roles of various epigenetic mechanisms, including DNA methylation, histone acetylation, imprinting, X-chromosome inactivation, telomere maintenance and expressions of development-related genes on somatic nuclear transfer.

作 者： LI Shijie Du Weihua LI Ning   作者單位： LI Shijie(State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China;Department of Biochemistry and Molecular Biology, College of Life Science, Hebei Agriculture University, Baoding 071001, China)

Du Weihua,LI Ning(State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China) 

刊 名： 科學通報（英文版）  SCI 英文刊名： CHINESE SCIENCE BULLETIN  年，卷(期)： 2004 49(8)  分類號： Q95  關鍵詞： somatic nuclear transfer   epigenetic reprogramming   DNA methylation   histon acetylation   X-chromosome activation   telomere   imprinting   gene expression