One New Method of Nucleic Acid Amplification-Loop-mediated Isothermal Amplification of DNA

Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method, which amplifies DNA with high specificity, sensitivity, rapidity and efficiency under isothermal conditions using a set of four specially designed primers and a Bst DNA polymerase with strand displacement activity. The basic principle, characteristics, development of LAMP and its applications are summarized in this article.

作 者： Xue-en FANG Jian LI Qin CHEN   作者單位： Xue-en FANG,Qin CHEN(School of Life Science, Shanghai University, Shanghai 200444, China)

Jian LI(Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China) 

刊 名： 中國病毒學  ISTIC 英文刊名： VIROLOGICA SINICA  年，卷(期)： 2008 23(3)  分類號： Q7  關鍵詞： Nucleic acid amplification   Loop-mediated isothermal amplification (LAMP)   Application  

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