Molecular genetic diversity of bacteria in the bottom section of arctic sea ice from the Canada Basin

PCR-DGGE approach was used to analyze bacterial diversity in the bottom section of seven arctic sea ice samples colleted from the Canada Basin. Thirty-two 16S rDNA sequences were obtained from prominent DGGE bands. The closest relatives of these sequences are found to be those of cultivated or uncultured bacteria from antarctic or arctic sea ice. Phylogenetic analysis clustered these sequences or phylotypes within α- proteobacteria, γ-proteobacteria and CFB (cytophaga-flexibacter-bacteroides) group. Sequences belonging to γ-proteobacteria were dominant and members of the CFB group were highly abundant. It was suggested that the CFB group was the representative of the bottom section of sea ice samples.

作 者： LI Huirong YU Yong CHEN Bo ZENG Yinxin REN Daming   作者單位： LI Huirong,YU Yong,CHEN Bo,ZENG Yinxin(State Oceanic Administration Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China)

REN Daming(State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433, China) 

刊 名： 海洋學報(英文版)  SCI 英文刊名： ACTA OCEANOLOGICA SINICA  年，卷(期)： 2005 24(6)  分類號： P7  關鍵詞： sea ice   Arctic Ocean   bacteria   16S rDNA   phylogenetic analysis  

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