Cloning, Expression and Characterization of Translationally Controlled Tumor Protein (TCTP) Gene from Flatfish Turbot (S

A full-length cDNA encoding translationally controlled tumor protein of marine flatfish turbot (Scophthalmus maximus), SmTCTP, was isolated with rapid amplification of cDNA Ends (RACE). SmTCTP consisted of a 5' untranslated region (UTR) of 84 bp, a 3' UTR of 451 bp and an open reading flame (ORF) of 513 bp, encoding a protein of 170 amino acid residues, which contained two signature sequences of TCTP family. The 5'UTR of SmTCTP started with a 5'-terminal oligopyrimidine tract (5'-TOP), a typical feature for translationaily controlled mRNAs. The deduced amino acid sequence of SmTCTP was similar to the other known verte-brate TCTPs in a range of 58.8% to 64.1%. The length offish TCTPs was diverse among species, e.g., TCTP of turbot and sea perch (Lateolabrax japonicus) is 170 aa in length, while that of zebrafish (Danio rer/o) and rohu (Labeo rohita) is 171 aa in length. North-ern blot analysis revealed that SmTCTP has only one type of mRNA. Its expression level in albino skin was slightly higher than that in normal skin. We constructed the pET3Oa-SmTCTP expression plasmid. The recombinant protein of His-tag SmTCTP was over-expressed in E. coli, purified and identified with peptide mass fingerprinting. These results may pave the way of further inves-tigation of the biological function of TCTP in fish.

作 者： WANG Jian GUO Huarong ZHANG Shicui YIN Licheng GUO Bin WANG Shaojie   作者單位： College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China  刊 名： 中國海洋大學學報（英文版）  ISTIC 英文刊名： JOURNAL OF OCEAN UNIVERSITY OF CHINA  年，卷(期)： 2008 7(2)  分類號： P7  關鍵詞： translationally controlled tumor protein (TCTP)   turbot   RACE   Northern blot   expression   peptide mass fingerprinting  

【Cloning, Expression and Characteriza】相關文章：

Cloning and Characterization of Porcine TSARG7 Gene and Analysis of Its Tissue-Specific Expression04-28

Human Cloning is Beneficial to Society05-04

Expression and role of Tie-2 in rectal carcinoma04-28

In Silico Cloning and Sequence Analysis of Phospholipase Dα Gene from Peach Fruit04-28

Cloning of the LFY cDNA from Arabidopsis thaliana and Its Transformation to Chrysanthemum morifolium04-27

Prokaryotic expression and characterization of a pea actin isoform (PEAcl) fused to GFP04-27

Expression of PHGPx in mammalian cells and its antiviral effect against coxsackievirus group B04-28

Cloning of a down-regulated gene encoding small GTP binding protein in hybrid wheat04-28

The Prokaryotic Expression and Bioactivity of the Recombinant Red Fire Ant Venom Allergen Soli404-29

Effect of RNA Interference Hsp72 Gene Expression on Development of Mouse Preimplantation Embryos04-29