Effects of Nutritional Factors on the Growth and Heterotrophic Eicosapentaenoic Acid Production of Diatom Nitzschia laev

The effects of several nutritional factors on the growth and eicosapentaenoic acid (EPA) production of diatom Nitzschia laevis were studied. 4 LDM (quadrupled concentration of the nutrient salt) was the optimal concentration of nutrient salt for the growth and EPA production ofN. laevis. The growth ofN. laevis was inhibited when the glucose concentration was either lower than 10gL-1 or higher than 15gL-1. Both sodium nitrate and urea were good nitrogen sources for the growth and EPA production, while ammonium chloride seriously decreased the dry cell weight (DW) and the EPA content. Silicate seriously influenced the growth of N. laevis. The maximum DW of 2.34gL-1 was obtained in the presence of 150 mgL-1 Na2SiO3·9H2O. The EPA content remained almost the same when the silicate concentration was lower than 150 mgL-1; however, higher silicate concentrations resulted in a steady de- crease of EPA content. Low medium salinity (≤29) did not seem to influence the DW of N. laevis, and high salinity resulted in a decrease of DW. The highest EPA content (4.08%) and yield (110 mgL-1) were observed at the salinity of 36 and 29, respectively.

作 者： CAO Xiaohong LI Songyao WANG Chunling LU Meifang   作者單位： Tianjin Key Laboratory of Food Nutrition and Safety, College of Food Science and Biotechnology, Tianfin University of Science and Technology, Tianjin 300457, P. R. China  刊 名： 中國海洋大學學報（英文版）  ISTIC 英文刊名： JOURNAL OF OCEAN UNIVERSITY OF CHINA  年，卷(期)： 2008 7(3)  分類號： P7  關鍵詞： polyunsaturated fatty acid   eicosapentaenoic acid   microalga   Nitzschia laevis   heterotrophy  

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