Comparison of Di-n-methyl Phthalate Biodegradation by Free and Immobilized Microbial Cells

Objective To compare the biodegradation of di-n-methyl pathalate by free and immobilizedmicrobial cells. Methods The enrichment and isolation technique was used to isolate themicroorganism. The PAV-entrapment method was utilized to immobilize the microorganisms. Thescanning electron microscophy (SEM) was used to observe the growth and distribution of microbialcells immobilized inside the PVA bead gels. The GC/MS method was used to identify the mainintermediates of DMP degradation. Results The microbial cells could grow quite well in PVA gel.The metabolic pathway did not change before and after immobilization of the microbial cells. Thedegradation rate of immobilized cells was higher than that of free cells. Conclusion Theimmobilized microbial cells possess advantages than free cells when applied to the biodegradation oftoxic organic pollutants.

作 者： JIAN-LONG WANG YU-CAI YE WEI-ZHONG WU   作者單位： JIAN-LONG WANG,YU-CAI YE(Laboratory of Environmental Technology, Institute of Nuclear Energy Technology, Tsinghua University, Beijing 100085, China)

WEI-ZHONG WU(School of Environment Science, Peking University, Beijing 100871, China) 

刊 名： 生物醫學與環境科學(英文版)  SCI 英文刊名： BIOMEDICAL AND ENVIRONMENTAL SCIENCES  年，卷(期)： 2003 16(2)  分類號： X1  關鍵詞： Priority pollutants   Phthalic acid ester   Immobilized microbial cells   Biodegradation