Optimization of solid fermentation of cellulase from Trichoderma koningii

Abstract： To exploit peashrub resources in Ordos as fodders, it is very crucial to realize industrial production of cheap cellulase of high activity by optimizing culture technology, especially culture substrate. In this study, a new prescription experiment based on uniform design ideal was invented and successfully applied in the solid fermentation of Trichoderma koningii F244, which being performed with two different temperature degrees. The activities of FPA, cotton lyase, CMCase and β-glucosidase were assayed and then mathematical models of enzymatic activities, which were figured out by Unconstraint Mathematical Programming, were developed by Multivariate Regression Program of SPSS10.0. Enzymatic activities of optimized substrate prescriptions corresponding to mathematical models were forecasted to determine an ideal substrate prescription. It is revealed that in solid fermentation, Tween80 has negative effect on cellulase production. Furthermore, the ideal prescription for cellulase complex production by Trichoderma koningii F244 was straw powder 16.9%,wheat bran 26.5%, (NH4)2SO4 9.5% and water 47.1%, whose corresponding cellulase activity was expected to be at the same high level with that of Trichoderma reesei Q9414 on its own recommended substrate. Especially, goats mainly fed on peashrub tissues mixed with cellulase complex of this prescription and culture technology, got an incremental ratio of 0.3 kg/d, which brought a very promising feeding prospect for local peashrub resource. By populization of this cellulase complex, it can integrate living standard, economic construction of local residents into vegetational restoration tightly and thus this paper will be very meaningful to be use for reference for western China like Ordos to realize its sustainable development of economy, society and environment. 作 者： LI Pei-jun    JING De-bing    ZHOU Qi-xing    ZHANG Chun-gui   作者單位： LI Pei-jun,ZHOU Qi-xing,ZHANG Chun-gui(Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China)

JING De-bing(Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China;Graduate School of the Chinese Academy of Sciences,Beijing 100039,China) 

期 刊： 環境科學學報（英文版）  ISTICSCI   Journal： JOURNAL OF ENVIRONMENTAL SCIENCES  年，卷(期)： 2004, 16(5)  分類號： X17  Keywords： Trichoderma koningii    substrate prescription    FPA    CMCase    cotton lyase    β-glucosidase   

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