Identification of an epitope of SARS-coronavirus nucleocapsid protein

The nucleocapsid (N) protein of severe acute respiratory syndrome-coronavirus (SARS-CoV) is a majorvirion structural protein. In this study, two epitopes (N1 and N2) of the N protein of SARS-CoV werepredicted by bioinformatics analysis. After immunization with two peptides, the peptides-specific antibodieswere isolated from the immunized rabbits. The further experiments demonstrated that N1 peptide-inducedpolyclonal antibodies had a high affinity to bind to E. coli expressed N protein of SARS-CoV. Furthermore, itwas confirmed that N1 peptide-specific IgG antibodies were detectable in the sera of severe acute respiratorysyndrome (SARS) patients. The results indicated that an epitope of the N protein has been identified andN protein specific Abs were produced by peptide immunization, which will be useful for the study of SARS-CoV.

作 者： Ying Lin XU Shen RUI FU YANG YI XUE LI YONG YONG JI YOU YU HE MUDE SHI WEI Lu TIE LIU SHI JIN WANG HONG XIA WANG HUA LIANG JIANG JIAN HUA SHEN YOU HUA XIE YUAN WANG GANG PEI BEI FEN SHEN JIA RUI WU Bing SUN   作者單位： Ying Lin,XU Shen,YI XUE LI,YONG YONG JI,YOU YU HE,MUDE SHI,WEI Lu,TIE LIU SHI,HUA LIANG JIANG,JIAN HUA SHEN,YOU HUA XIE,YUAN WANG,GANG PEI,JIA RUI WU,Bing SUN(Institute of Biochemistry and Cell Biology, Institute of Materia Medica, Bioinformation Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China)

RUI FU YANG,JIN WANG,HONG XIA WANG,BEI FEN SHEN(Institute of Microbiology and Epidemiology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100071, China) 

刊 名： 細胞研究(英文版)  ISTIC SCI 英文刊名： CELL RESEARCH  年，卷(期)： 2003 13(3)  分類號： Q2  關鍵詞： severe acute respiratory syndrome-coronavirus   necleocapsid protein   epitope   polyclonal antibody   antiserum  

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