Alpha-picolinic acid,a fungal toxin and mammal apoptosis-inducing agent,elicits hypersensitive-like response and enhance

Alpha-picolinic acid (PA),a metabolite of tryptophan and an inducer of apoptosis in the animal cell,has been reported to be a toxin produced by some of plant fungal pathogens and used in screening for disease resistant mutants. Here,we report that PA is an efficient apoptosis agent triggering cell death of hypersensitive-like response in planta. Confirmed by Fluorescence Activated Cell Sorter (FACS),rice suspension cells and leaves exhibited programmed cell death induced by PA. The PA-induced cell death was associated with the accumulation of reactive oxygen species that could be blocked by diphenylene iodonium chloride,indicating that the generation of reactive oxygen species was NADPHoxidase dependent. We also demonstrated the induction of rice defense-related genes and subsequent resistant enhancement by PA against the rice blast fungus Magnaporthe grisea. Hence,it was concluded that the PA-stimulated defense response likely involves the onset of the hypersensitive response in rice,which also provides a simple eliciting tool for studying apoptosis in the plant cell.

作 者： Hai Kuo ZHANG Xin ZHANG Bi Zeng MAO Qun Li Zu Hua HE   作者單位： Hai Kuo ZHANG,Xin ZHANG,Qun Li(SHARF and National Laboratory of Plant Molecular Genetics,Shanghai Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai,200032,China)

Bi Zeng MAO(Biotechnology Institute,Zhejiang University,Hangzhou,310029,China)

Zu Hua HE(SHARF and National Laboratory of Plant Molecular Genetics,Shanghai,Institute of Plant Physiology and Ecology,Shanghai,Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai,200032,China;Biotechnology Institute,Zhejiang University,Hangzhou) 

刊 名： 細胞研究(英文版)  ISTIC SCI 英文刊名： CELL RESEARCH  年，卷(期)： 2004 14(1)  分類號： Q2  關鍵詞： picolinic acid   cell death   hypersensitive responses   Magraporche grisea   rice