Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia

A study was conducted to improve 9-methoxycanthin-6-one productivity (potential anti-tumour compound) from callus cultures of Eurycoma longifolia (Tongkat Ali). Several factors affecting 9-methoxycanthin-6-one production in callus cultures such as different medium compositions and physical factors were investigated and analyzed. Results show that a higher production of 9-methoxycanthin-6-one (3.84 mg(g-1 DW (Dry Weight)) is obtained from callus cultured in 1/4 MS basal media. At fructose of 2% (w/v), the production of 9-methoxycanthin-6-one (4.59 mg(g-1 DW) is promoted to gain the highest yield, compared to other carbon sources tested. The addition of 2.0-mg(L-1 dicamba also increases 9-methoxycanthin-6-one production (12.3 mg(g-1 DW). Higher production of 9-methoxycanthin-6-one was obtained at pH 5.5 (1.53 mg(g-1 DW). Production of 9-methoxycanthin-6-one (2.34 mg(g-1 DW) in callus cultures is also increased when the medium is added with 1(10-1 μM phenylalanine. This study suggests that the successful production of 9-methoxycanthin-6-one in vitro cultures has a potential in large-scale production using bioreactor technology.

作 者： N. Rosli M. Maziah K. L. Chan S. Sreeramanan   作者單位： N. Rosli(Department of Applied Science, Universiti Teknologi MARA Negeri Sembilan, Kuala Pilah 72000, Negeri Sembilan Darul Khusus, Malaysia;Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Malaysia)

M. Maziah(Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Malaysia)

K. L. Chan(School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden Heights, Penang 11800, Malaysia)

S. Sreeramanan(Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Malaysia;School of Biological Sciences, Universiti Sains Malaysia, Minden Heights, Penang 11800, Malaysia) 

刊 名： 林業研究（英文版）  英文刊名： JOURNAL OF FORESTRY RESEARCH  年，卷(期)： 2009 20(1)  分類號： S7  關鍵詞： Eurycoma longifolia   callus culture   9-methoxycanthin-6-one   dry weight  

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